Embedded Files
Recombinant Human Siglec-8 V-set, His Tag
last updated: Oct 28, 2025
Key features
Key features
Expression system: Escherichia coli
Purity: > 90% SDS-PAGE
Active: Yes
Tags: His tag C-Terminus
Validated for: Lectin Blotting
Description
Description
Synonyms
SIGLEC8, Sialic acid-binding Ig-like lectin 8, Sialoadhesin family member 2, SAF2
Source
Escherichia coli-derived human Siglec 8 protein V-set domain
Expression host
Escherichia coli
Sequence
Glu18-His155
Accession
Molecular weight
17.1 kDa
Tag
C-terminus His-tag
Properties
Properties
Purity
> 90 % as determined by reducing SDS-PAGE.
Endotoxin level
< 1 EU/μg determined by the LAL method
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months, -20 to -70 °C in lyophilized state.
- 1 week, 2 to 8 °C under sterile conditions after reconstitution.
Shipping
This product is shipped with dry ice.
Formulation
Lyophilized from filtered 20 mM potassium phosphate pH 7.0, supplemented with 40 mM NaCl, 5% trehalose, and 1 mM DTT
Reconstitution
Reconstitute at 500 μg/mL in sterile deionized water.
Activity validation
Measured by its ability to distinguish fetuin and asialofetuin in a lectin blotting assay using a concentration of 10 μg/mL.
Bioactivities validated by GlycoGenetics, Inc.
Bioactivities validated by GlycoGenetics, Inc.
Lectin blotting
Lectin blotting
Siglec-8 V-set shows sialic acid-dependent binding at 20 μg/mL, as determined by lectin blotting.
Page updated
Google Sites
Report abuse