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Recombinant Human Siglec-7, His Tag
Key features
Key features
Expression system: Escherichia coli
Purity: > 90% SDS-PAGE
Active: Yes
Tags: His tag C-Terminus
Suitable for: Functional Studies, SDS-PAGE
Description
Description
Synonyms
Sialic acid-binding Ig-like lectin 7, SIGLEC7, AIRM1
Source
Escherichia coli-derived human Siglec 7 protein V-set domain
Expression_host
Escherichia coli
Sequence
Gln19-Ala145
Accession
Q9Y286
Mol_Mass
15.8 kDa
Tag
C-terminus His-tag
Bio_Activity
Measured by its ability to interact with fixed K562 cell using a concentration range of 2.0-25 μg/mL.
Properties
Properties
Purity
> 90 % as determined by reducing SDS-PAGE.
Endotoxin level
< 1 EU/μg determined by the LAL method
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 week, 2 to 8 °C under sterile conditions after reconstitution.
- 1 month, -20 to -70 °C under sterile conditions after reconstitution.
(The stability trail is still ongoing)
Shipping
This product is shipped with dry ice.
Formulation
Lyophilized from filtered 20 mM potassium phosphate (pH 7.0), 40mM NaCl, 5% trehalose, 1 mM DTT.
Reconstitution
Reconstitute at 500 μg/mL in sterile deionized water.
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