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Recombinant Human Siglec-7 V-set, His Tag
last updated: Oct 28, 2025
Key features
Key features
Expression system: Escherichia coli
Purity: > 90% SDS-PAGE
Active: Yes
Tags: His tag C-Terminus
Validated for: Lectin Blotting, Flow Cytometry Analysis
Description
Description
Synonyms
Sialic acid-binding Ig-like lectin 7, SIGLEC7, AIRM1
Source
Escherichia coli-derived human Siglec 7 protein V-set domain
Expression host
Escherichia coli
Sequence
Gln19-Ala145
Accession
Molecular weight
15.8 kDa
Tag
C-terminus His-tag
Properties
Properties
Purity
> 90 % as determined by reducing SDS-PAGE.
Endotoxin level
< 1 EU/μg determined by the LAL method
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months, -20 to -70 °C in lyophilized state.
- 1 week, 2 to 8 °C under sterile conditions after reconstitution.
Shipping
This product is shipped with dry ice.
Formulation
Lyophilized from filtered 20 mM potassium phosphate pH 7.0, supplemented with 40 mM NaCl, 5% trehalose, and 1 mM DTT.
Reconstitution
Reconstitute at 500 μg/mL in sterile deionized water.
Activity validation
Measured by its ability to distinguish fetuin and asialofetuin in a lectin blotting assay using a concentration of 5 μg/mL.
Bioactivities validated by GlycoGenetics, Inc.
Bioactivities validated by GlycoGenetics, Inc.
Lectin blotting
Lectin blotting
Siglec-7 V-set shows sialic acid-dependent binding at 5 μg/mL, as determined by lectin blotting.
Cell binding by flow cytometry
Cell binding by flow cytometry
Siglec-7 V-set demonstrates binding to fixed K562 cells at 5 and 10 μg/mL concentrations, as determined by flow cytometry.
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