Products

Synonyms

Galectin-3, LGALS3, MAC2, Gal-3, 35 kDa lectin, CBP 35, GALBP, IgE-binding protein, L-31, Laminin-binding protein, Lectin L-29, Mac-2 antigen

Source

Escherichia coli-derived human Galectin 3 protein

Expression host　

Escherichia coli

Sequence　

Met1-Ile250

Accession　

P17931

Molecular weight

27 kDa

Tag　

C-terminus His-tag

Purity

> 90% as determined by reducing SDS-PAGE.

Endotoxin level

< 1 EU/μg determined by the LAL method

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months, -20 to -70 °C in lyophilized state.
- 1 week, 2 to 8 °C under sterile conditions after reconstitution. 

Shipping

This product is shipped with dry ice.

Formulation

Lyophilized from filtered PBS pH 7.4, supplemented with 363 mM NaCl, 5% sucrose, and 1 mM DTT.

Reconstitution

Reconstitute at 500 μg/mL in sterile deionized water.

Activity validation

Measured by its ability to interact with fixed Jurkat cells at a concentration of 2.0 or 25 μg/mL.

Cell binding by flow cytometry

Gal-3 demonstrates dose-dependent binding to fixed K562 cells within the 0-20 μg/mL concentration range, as determined by flow cytometry.