Embedded Files
Key features
Key features
Expression system: Escherichia coli
Purity: > 90% SDS-PAGE
Active: Yes
Tags: His tag C-Terminus
Validated for: Flow Cytometry Analysis
Description
Description
Synonyms
LGALS1, GAL1, GBP, Galectin-1
Source
Escherichia coli-derived human Galectin 1 protein
Expression host
Escherichia coli
Sequence
Met1-Asp135
Accession
Molecular weight
15.5 kDa
Tag
C-terminus His-tag
Properties
Properties
Purity
> 90 % as determined by reducing SDS-PAGE.
Endotoxin level
< 2.5 EU/μg determined by the LAL method
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months, -20 to -70 °C in lyophilized state.
- 1 week, 2 to 8 °C under sterile conditions after reconstitution.
Shipping
This product is shipped with dry ice.
Formulation
Lyophilized from filtered PBS pH 7.4, supplemented with 1 mM DTT, 5% Trehalose, and 10 mM Glutamic acid.
Reconstitution
Reconstitute at 500 μg/mL in sterile deionized water.
Activity validation
Measured by its ability to interact with fixed Jurkat cells using a concentration of 2.0 or 25 μg/mL.
Bioactivities validated by GlycoGenetics, Inc.
Bioactivities validated by GlycoGenetics, Inc.
Cell binding by flow cytometry
Cell binding by flow cytometry
Gal-1 demonstrates dose-dependent binding to fixed K562 cells within the 0-50 μg/mL concentration range, as determined by flow cytometry.
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