Currently Available Lectins

Fixed K562 cells were stained by FITC-labeled recombinant lectins, and the mixture was subjected to flow cytometry analysis on a CytoFlexS Flow Cytometer. FITC-labeled BSA was used as a background control. Relative MFI change was calculated by dividing the lectin-binding MFI value of each mutant by that of the wildtype. All the cells used were derived from K562 with fixation by paraformaldehyde solution. All measurements were carried in duplicate.